| Source: |
Escherichia coli |
| Molecular Weight: |
Approximately 9.4 kDa, a single non-glycosylated polypeptide chain containing 77amino acids. |
| AA Sequence: |
SKCKCSRKGP KIRYSDVKKL EMKPKYPHCE EKMVIVTTKS MSRYRGQEHC LHPKLQSTKR FIKWYNAWNE KRRVYEE |
| Purity: |
> 95% by SDS-PAGE and HPLC analyses. |
| Biological Activity: |
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human monocytes is in a concentration range of 1.0-10 ng/mL. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, 400 mM NaCl, pH7.4, 5% trehalose. |
| Endotoxin: |
Less than 1 EU/μg of rMuBRAK/CXCL14 as determined by LAL method. |
| Reconstitution: |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤-20°C. Further dilutions should be made in appropriate buffered solutions. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Synonyms: |
B-cell and Monocyte-activating Chemokine, Chemokine BRAK, Kidney-expressed Chemokine CXC, MIP-2G, Small-inducible Cytokine B14 |
| Background: |
Chemokine (C-X-C motif) ligand 14 (CXCL14), also known as BRAK, is a small cytokine belonging to the CXC chemokine family. It is constitutively expressed at the mRNA level in specific normal tissues and exhibits chemoattractive properties towards activated macrophages, immature dendritic cells, and natural killer cells. CXCL14 has been implicated in tumor development according to existing literature. Recent studies also highlight its involvement in glucose metabolism, feeding behavior-related neuronal circuits, and antimicrobial defense mechanisms.
Despite the lack of identified CXCL14 receptors, its intracellular activity in breast cancer cells suggests that its receptor(s) and signaling pathways may differ from those typically associated with conventional CXC chemokines. Recombinant murine CXCL14 comprises 77 amino acid residues and shares high sequence identities of 97% and 99% with human and rat CXCL14, respectively. |
