| Source: |
Escherichia coli. |
| Molecular Weight: |
Approximately 16.5 kDa, a single non-glycosylated polypeptide chain containing 146 amino acids. |
| AA Sequence: |
MPALPEDGGA AFPPGHFKDP KRLYCKNGGF FLRIHPDGRV DGVREKSDPH VKLQLQAEER GVVSIKGVCA NRYLAMKEDG RLLASKCVTE ECFFFERLES NNYNTYRSRK YSSWYVALKR TGQYKLGSKT GPGQKAILFL PMSAKS |
| Purity: |
> 98% by SDS-PAGE and HPLC analyses. |
| Biological Activity: |
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine balb/c 3T3 cells is less than 1.0 ng/mL, corresponding to a specific activity of > 1.0 × 106 IU/mg. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from a 0.2 µm filtered solution in PBS, pH7.4. |
| Endotoxin: |
Less than 1 EU/µg of rMubFGF as determined by LAL method. |
| Reconstitution: |
Centrifuge the vial briefly before opening to ensure that the contents settle at the bottom. Reconstitute the vial with sterile distilled water or an aqueous buffer containing 0.1% BSA to achieve a concentration of 0.1-1.0 mg/mL. Divide the resulting stock solution into working aliquots and store them at or below -20°C. For further dilutions, use appropriate buffered solutions. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Synonyms: |
FGF-2, HBGF-2 |
| Background: |
Murine bFGF, manufactured by the FGF2 gene, is part of the fibroblast growth factor (FGF) family. Fibroblast growth factor was uncovered in pituitary extracts in 1973 and tested in a bioassay that led to fibroblasts proliferation. After further fractionating the extract using acidic and basic pH, "acidic fibroblast growth factor" (FGF-1) and "basic fibroblast growth factor" (FGF-2) were isolated. The affinity between bFGF and its receptors can be increased by heparin or heparan sulfate proteoglycan. Murine bFGF shares a 95% a.a. sequence identity with human bFGF. |
