| Source: |
Insect Cell |
| Molecular Weight: |
Approximately 26.0 kDa, a homodimeric protein consisting of two 116 amino acid polypeptide chains. |
| AA Sequence: |
GLECDGKVNI CCKKQFFVSF KDIGWNDWII APSGYHANYC EGECPSHIAG TSGSSLSFHS TVINHYRMRG HSPFANLKSC CVPTKLRPMS MLYYDDGQNI IKKDIQNMIV EECGCS |
| Purity: |
> 95% by SDS-PAGE analyses. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM Tris-HCl, pH8.0, 300 mM NaCl, with 5% Trehalose, 0.02% Tween-20. |
| Endotoxin: |
Less than 0.1 EU/μg of rHu/Bo/Mu/Rt Activin A/Inhibin beta A, Insect Cell as determined by LAL method. |
| Reconstitution: |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile 10mM HCl to a concentration of 0.1-0.3 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Background: |
Activin and inhibin are members of the TGF-beta superfamily and play roles in tissue morphogenesis, repair, fibrosis, inflammation, neural development, hematopoiesis, reproductive function, and carcinogenesis. Produced as precursor proteins, their propeptides are cleaved to form bioactive disulfide-linked dimers. Activins are homodimers or heterodimers of various beta subunits (beta A, B, C, E), while inhibins are heterodimers of an alpha subunit and a beta subunit. Activin A, a widely expressed homodimer of two beta A chains, can also form Activin AB and Activin AC. Activin A binds to Activin RIIA, which associates with Activin RIB/ALK-4, leading to Smad activation and gene transcription regulation. Its bioactivity is controlled by mechanisms involving BAMBI, Betaglycan, and Cripto, which act as decoy receptors, and by forming inactive complexes with alpha 2-Macroglobulin, Follistatin, and FLRG. |
