| Synonym(s): |
MGC119418; MGC119419; MGC119420; protein Wnt-3a; wingless-type MMTV integration site family, member 3A; Wnt3a; Wnt-3a |
| Purity: |
> 75%, by SDS-PAGE under reducing conditions and visualized by silver stain. |
| Biological Activity: |
Measured by its ability to induce alkaline phosphatase production by MC3T3‑E1 mouse preosteoblast cells. The ED50 for this effect is 5.00-25.0 ng/mL. Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The ED50 for this effect is <500 ng/mL. Protein concentrations should be titrated based on cell type and if appropriate, passage number of the cell line.
Optimal concentrations should be determined by each laboratory for each application. |
| Endotoxin: |
< 1.0 EU per 1 μg of the protein by the LAL method. |
| Predicted Molecular Mass: |
37.4 kDa |
| Form: |
Lyophilized from a 0.2 μm filtered solution in PBS, EDTA and CHAPS. |
| Shipping Condition: |
Shipping at ambient temperature. |
| Storage Conditions: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Source: |
Hamster Ovary cell line, CHO-derived human Wnt-3a protein
Ser19-Lys352. |
| Reconstitution: |
Reconstitute at 200 μg/mL in sterile PBS. |
