| Source: |
Escherichia coli |
| Molecular Weight: |
Approximately 13.2 kDa, a homodimeric protein consisting of two 59 amino acid chains, which includes a 40-amino acid trefoil motif containing three conserved intramolecular disulfide bonds. |
| AA Sequence: |
EEYVGLSANQ CAVPAKDRVD CGYPHVTPKE CNNRGCCFDS RIPGVPWCFK PLQEAECTF |
| Purity: |
> 97% by SDS-PAGE and HPLC analyses. |
| Biological Activity: |
Fully biologically active when compared to standard. The ED50 as determined by a chemotaxis bioassay using human MCF-7 cells is less than 10 μg/mL, corresponding to a specific activity of > 100 IU/mg. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH7.4. |
| Endotoxin: |
Less than 1 EU/μg of rHuTFF3 as determined by LAL method. |
| Reconstitution: |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Synonyms: |
Intestinal Trefoil Factor, hITF, Polypeptide P1.B, hP1.B |
| Background: |
Trefoil factor 3 (TFF3), encoded by the TFF3 gene in humans, is part of the trefoil factor family consisting of TFF1, TFF2, and TFF3. These proteins are characterized by containing at least one trefoil motif, a 40-amino acid domain with three conserved disulfide bonds. TFF3 is primarily expressed by goblet cells and in the uterus. It has also been observed in various cancers such as colorectal, hepatocellular, and biliary tumors.
TFF3 plays a role in maintaining and repairing the intestinal mucosa and promotes epithelial cell mobility during the healing process. Overexpression of TFF3 is implicated in the progression of hepatocellular carcinoma in both mice and humans. TFF3 has potential as a molecular marker for certain cancers; however, its precise role in tumorigenesis remains unclear. |
