| Source: |
Escherichia coli |
| Molecular Weight: |
Approximately 12.0 kDa, a single non-glycosylated polypeptide chain containing 106 amino acids, which includes a 40-amino acid trefoil motif containing three conserved intramolecular disulfide bonds. |
| AA Sequence: |
EKPSPCQCSR LSPHNRTNCG FPGITSDQCF DNGCCFDSSV TGVPWCFHPL PKQESDQCVM EVSDRRNCGY PGISPEECAS RKCCFSNFIF EVPWCFFPKS VEDCHY |
| Purity: |
> 97% by SDS-PAGE and HPLC analyses. |
| Biological Activity: |
Fully biologically active when compared to standard. The ED50 as determined by a chemotaxis bioassay using human MCF-7 cells is less than 10 ng/mL, corresponding to a specific activity of > 1.0 × 105 IU/mg. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from a 0.2 µm filtered concentrated solution in 20 mM PB, pH7.4, 130 mM NaCl. |
| Endotoxin: |
Less than 1 EU/µg of rHuTFF2 as determined by LAL method. |
| Reconstitution: |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Synonyms: |
Spasmolysin, Spasmolytic polypeptide, SP |
| Background: |
Trefoil factor 2 (TFF2), encoded by the TFF2 gene in humans, is a member of the trefoil factor family alongside TFF1 and TFF3. These proteins are distinguished by containing the trefoil motif, a 40-amino acid domain with three conserved disulfide bonds. TFFs are robust secretory proteins prominently expressed in the gastrointestinal tract, particularly in the gastric mucosa. TFF2 plays a role in inhibiting gastrointestinal motility and gastric acid secretion. Moreover, it serves as a structural component of gastric mucus, potentially enhancing the stability of glycoproteins within the mucus gel through interactions with carbohydrate side chains. |
