| Source: |
Escherichia coli |
| Molecular Weight: |
Approximately 4.9 kDa, a single non-glycosylated polypeptide chain containing 43 amino acids. |
| AA Sequence: |
SDKPDMAEIE KFDKSKLKKT ETQEKNPLPS KETIEQEKQA GES |
| Purity: |
> 97% by SDS-PAGE and HPLC analyses. |
| Biological Activity: |
Fully biologically active when compared to standard. The biological activity determined by its ability to produce a protective effect against hydrogen peroxide in primary lung fibroblasts is in a concentration range of 0.5 - 10 μg/mL. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from a 0.2 µm filtered concentrated solution in 20 mM PB, pH7.4. |
| Endotoxin: |
Less than 1 EU/µg of rHuTβ4 as determined by LAL method. |
| Reconstitution: |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Synonyms: |
Fx |
| Background: |
Thymosin beta 4, a peptide naturally produced from the TMSB4X gene on human chromosome X, is present in significant amounts in blood platelets, wound fluid, and various tissues. It primarily regulates actin and stimulates the production of T cells, crucial for immune function. When administered post-heart attack, Thymosin beta 4 may potentially activate cardiac progenitor cells to facilitate the repair of damaged heart tissue. |
