| Source: |
Escherichia coli |
| Molecular Weight: |
Approximately 13.9 kDa, a single non-glycosylated polypeptide chain containing 127 amino acids. But it migrates with an apparent molecular mass of 22 kDa in SDS-PAGE. |
| AA Sequence: |
ESIRETEVID PQDLLEGRYF SGALPDDEDV VGPGQESDDF ELSGSGDLDD LEDSMIGPEV VHPLVPLDNH IPERAGSGSQ VPTEPKKLEE NEVIPKRISP VEESEDVSNK VSMSSTVQGS NIFERTE |
| Purity: |
> 95% by SDS-PAGE and HPLC analyses. |
| Biological Activity: |
Fully biologically active when compared to standard. The specific activity is determined by binding ability in a functional ELISA. Immobilized rHuSYND4 at 500 ng/mL (100 μl/well) can bind rHubFGF with a linear range of 0.1-10 ng/mL. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH7.4. |
| Endotoxin: |
Less than 0.1 EU/μg of rHuSYND4 as determined by LAL method. |
| Reconstitution: |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Background: |
Syndecan-4 (SYND4), encoded by the SDC-4 gene in humans, weighs approximately 20 kDa. It belongs to the syndecan family of Type 1 transmembrane proteins, alongside three other vertebrate syndecans. Syndecans are known for their ability to carry heparan sulfate (HS) and chondroitin sulfate glycosaminoglycans. They are characterized by two conserved cytoplasmic domains and variable extracellular regions, except for the sites where HS attaches.
SYND4 bears the closest resemblance to SYND2 but exhibits broader expression across nearly all cell types. Its expression can be induced by TGFβ2 and in response to mechanical stress, seen notably in smooth muscle, wound healing, arterial injury, and acute myocardial infarction, likely triggered by inflammatory mediators. Unlike other syndecans, SYND4 has a widespread distribution and consistently appears in focal adhesions.
The extracellular domain (ECD) of human SYND4 shares approximately 79%, 78%, and 81% amino acid identity with its counterparts in mouse, rat, and porcine species, respectively. |
