| Source: |
Escherichia coli. |
| Molecular Weight: |
Approximately 8.5 kDa, a single non-glycosylated polypeptide chain containing 72 amino acid residues. |
| AA Sequence: |
KPVSLSYRCP CRFFESHVAR ANVKHLKILN TPNCALQIVA RLKNNNRQVC IDPKLKWIQE YLEKALNKRF KM |
| Purity: |
> 97% by SDS-PAGE and HPLC analyses. |
| Biological Activity: |
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using PHA and rHuIL-2 activated human peripheral blood T-lymphocytes is in a concentration range of 20-80 ng/mL. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH7.4. |
| Endotoxin: |
Less than 1 EU/μg of rHuSDF-1β/CXCL12β as determined by LAL method. |
| Reconstitution: |
Centrifuge the vial briefly before opening to ensure that the contents settle at the bottom. Reconstitute the vial with sterile distilled water or an aqueous buffer containing 0.1% BSA to achieve a concentration of 0.1-1.0 mg/mL. Divide the resulting stock solution into working aliquots and store them at or below -20°C. For further dilutions, use appropriate buffered solutions. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Synonyms: |
hSDF-1 beta, IRH, hIRH, PBSF |
| Background: |
CXCL12, also referred to as SDF-1, is a member of the CXC chemokine family with its encoding done by the CXCL12 gene. Recent studies show that human CXCL12 is represented in six isoforms, each differing solely at the C-terminal tail. Every SDF-1 isoform experiences proteolytic processing of the first two N-terminal amino acids. Among all these isoforms, SDF-1β boasts the canonical sequence, containing the complete C-terminal tail's amino acids. The chemokine's receptor on the cell surface is CXCR4 and syndecan4, with CXCL12 exerting a strong chemotactic effect on T-lymphocytes and monocytes, but not on neutrophils. In addition, CXCL12 serves a crucial role in both carcinogenesis and the neovascularisation associated with tumor growth. |
