| Source: |
Escherichia coli |
| Molecular Weight: |
Approximately 36.0 kDa, a single non-glycosylated polypeptide chain containing 330 amino acids, including the 317 residues of full-length Sox2 and a 13-residue C-terminal TAT peptide (GGYGRKKRRQRRR). |
| AA Sequence: |
MYNMMETELK PPGPQQTSGG GGGNSTAAAA GGNQKNSPDR VKRPMNAFMV WSRGQRRKMA QENPKMHNSE ISKRLGAEWK LLSETEKRPF IDEAKRLRAL HMKEHPDYKY RPRRKTKTLM KKDKYTLPGG LLAPGGNSMA SGVGVGAGLG AGVNQRMDSY AHMNGWSNGS YSMMQDQLGY PQHPGLNAHG AAQMQPMHRY DVSALQYNSM TSSQTYMNGS PTYSMSYSQQ GTPGMALGSM GSVVKSEASS SPPVVTSSSH SRAPCQAGDL RDMISMYLPG AEVPEPAAPS RLHMSQHYQS GPVPGTAING TLPLSHMGGY GRKKRRQRRR |
| Purity: |
> 95% by SDS-PAGE and HPLC analyses. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from a 0.2 μm filtered concentrated solution in 2 × PBS, pH7.4, with 5% trehalose. |
| Endotoxin: |
Less than 1 EU/μg of rHuSOX2-TAT as determined by LAL method. |
| Reconstitution: |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Synonyms: |
SOX2-TAT |
| Background: |
Sox2, also known as SRY (sex determining region Y)-box 2, belongs to the Sox family of proteins encoded by the Sox gene in humans. This protein family features highly conserved DNA binding domains called HMG (High-mobility group) box domains, each approximately 80 amino acids in length. Sox2 is crucial for maintaining embryonic and neural stem cells, making it pivotal in research on induced pluripotency, a burgeoning field in regenerative medicine. The mature human Sox2 protein shares 100% amino acid sequence identity with its counterparts in mice and rats. Recombinant human Sox2-TAT, expressed in E. coli, is a 36 kDa protein comprising 330 amino acids. This includes the full-length Sox2 sequence of 317 residues and a 13-residue C-terminal TAT peptide (GGYGRKKRRQRRR). |
