| Source: |
Escherichia coli |
| Molecular Weight: |
Approximately 19.8 kDa, a single non-glycosylated polypeptide chain containing 176 amino acids. |
| AA Sequence: |
IVIGPGRGFG KRRHPKKLTP LAYKQFIPNV AEKTLGASGR YEGKISRNSE RFKELTPNYN PDIIFKDEEN TGADRLMTQR CKDKLNALAI SVMNQWPGVK LRVTEGWDED GHHSEESLHY EGRAVDITTS DRDRSKYGML ARLAVEAGFD WVYYESKAHI HCSVKAENSV AAKSGG |
| Purity: |
> 98% by SDS-PAGE and HPLC analyses. |
| Biological Activity: |
Fully biologically active when compared to standard. The ED50 as determined by inducing alkaline phosphatase production of murine C3H/10T1/2 cells is less than 1 μg/mL, corresponding to a specific activity of > 1.0 × 103 IU/mg. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from a 0.2 µm filtered concentrated solution in 20 mM PB, pH7.4, 150 mM NaCl. |
| Endotoxin: |
Less than 1 EU/µg of rHuSHH as determined by LAL method. |
| Reconstitution: |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Synonyms: |
HHG-1 |
| Background: |
Sonic Hedgehog (SHH) belongs to the Hedgehog (Hh) protein family, which also includes Desert Hedgehog (DHH) and Indian Hedgehog (IHH). These proteins share a high sequence similarity; for instance, SHH and IHH are 93% identical. SHH expression occurs primarily in the fetal intestine, liver, lung, and kidney, rather than in adult tissues. Structurally, the protein consists of 462 amino acids, including a 23-amino acid signal peptide at the N-terminus. It undergoes cleavage to form SHH N-Terminus and C-Terminus fragments.
SHH plays crucial roles in development, functioning as a morphogen that regulates the patterning of various systems such as the limb, midline structures of the brain and spinal cord, the thalamus via the zona limitans intrathalamica, and tooth development. In the absence of Sonic Hedgehog, the patched receptor suppresses the constitutive signaling activity of smoothened.
SHH-N, which retains all known signaling capabilities, can undergo lipid modification without losing its receptor affinity, resulting in a more potent form than the unmodified protein. Recombinant human SHH (rHuSHH) substitutes the natural chemically modified Cysteine residue with an N-terminal Ile-Val-Ile sequence. |
