Recombinant Human Soluble CD40 Ligand/TNFSF5

2-1-1-green-tea-extract-1

Recombinant Human Soluble CD40 Ligand/TNFSF5

Cat. No.: SPODRP01573
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Product Details

Source: Escherichia coli.
Molecular Weight: Approximately 16.3 kDa, a single non-glycosylated polypeptide chain containing 149 amino acids.
AA Sequence: MQKGDQNPQI AAHVISEASS KTTSVLQWAE KGYYTMSNNL VTLENGKQLT VKRQGLYYIY AQVTFCSNRE ASSQAPFIAS LWLKSPGRFE RILLRAANTH SSAKPCGQQS IHLGGVFELQ PGASVFVNVT DPSQVSHGTG FTSFGLLKL
Purity: > 95% by SDS-PAGE and HPLC analyses.
Biological Activity: Fully biologically active when compared to standard. The ED50 as determined by the dose-dependent stimulation of IL-8 production by human PBMC is less than 5-10 ng/mL.
Physical Appearance: Sterile filtered white lyophilized (freeze-dried) powder.
Formulation: Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH7.0.
Endotoxin: Less than 1 EU/μg of rHusCD40L as determined by LAL method.
Reconstitution: Centrifuge the vial briefly before opening to ensure that the contents settle at the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-0.3 mg/mL. Divide the resulting stock solution into working aliquots and store them at or below -20°C. For further dilutions, use appropriate buffered solutions.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution.
Synonyms: TNFSF5, sCD40L, CD154, gp39, T-BAM, TNF-related Activation Protein, TRAP
Background: The CD40 ligand is a type II transmembrane glycoprotein consisting of 261 amino acids and is a member of the TNF family. It can split into a membrane-bound form and a soluble form. This ligand is mainly found on activated CD4+ T lymphocytes but also appears in other cell types such as NK cells, mast cells, basophils, and eosinophils. It has three known binding partners: CD40, α5β1 integrin, and αIIbβ3. The CD40 ligand is instrumental in various B cell functions, including the activation of surface antigens, progression into the cell cycle, isotype switching, Ig secretion, and memory formation. The interaction between CD40 and CD40L is also crucial for monocyte activation and the maturation of dendritic cells.

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