| Source: |
Escherichia coli. |
| Molecular Weight: |
Approximately 7.8 kDa, a single non-glycosylated polypeptide chain containing 68 amino acids. |
| AA Sequence: |
SPYSSDTTPC CFAYIARPLP RAHIKEYFYT SGKCSNPAVV FVTRKNRQVC ANPEKKWVRE YINSLEMS |
| Purity: |
> 98% by SDS-PAGE and HPLC analyses. |
| Biological Activity: |
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood monocytes is in a concentration range of 1.0-10 ng/mL. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from a 0.2 μm filtered concentrated solution in 20mM PB, pH7.4, 100 mM NaCl. |
| Endotoxin: |
Less than 1 EU/μg of rHuRANTES/CCL5 as determined by LAL method. |
| Reconstitution: |
Centrifuge the vial briefly before opening to ensure that the contents settle at the bottom. Reconstitute the vial with sterile distilled water or an aqueous buffer containing 0.1% BSA to achieve a concentration of 0.1-1.0 mg/mL. Divide the resulting stock solution into working aliquots and store them at or below -20°C. For further dilutions, use appropriate buffered solutions. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Synonyms: |
SIS-delta, Small-inducible cytokine A5, T-cell-specific protein RANTES |
| Background: |
Classified as a chemotactic cytokine, Chemokine (C-C motif) ligand 5 (CCL5), also referred to as RANTES, is produced by the CCL5 gene in humans. It directs T cells, eosinophils, and basophils to inflammatory sites and is known to be secreted by renal tubular epithelium, synovial fibroblasts, and certain tumor cells. It also promotes the proliferation and activation of specific NK cells to form CHAK cells. The recombinant form of human CCL5 consists of 68 amino acids and shares 84% amino acid sequence identity with both murine and rat CCL5. |
