| Source: |
Escherichia coli. |
| Molecular Weight: |
Approximately 7.8 kDa, a single non-glycosylated polypeptide chain containing 70 amino acids. |
| AA Sequence: |
EAEEDGDLQC LCVKTTSQVR PRHITSLEVI KAGPHCPTAQ LIATLKNGRK ICLDLQAPLY KKIIKKLLES |
| Purity: |
> 97% by SDS-PAGE and HPLC analyses. |
| Biological Activity: |
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human fibroblasts is in a concentration of 1.0-10 ng/mL. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from a 0.2 μm filtered concentrated solution in Acetonitrile and TFA. |
| Endotoxin: |
Less than 1 EU/μg of rHuPF-4/CXCL4 as determined by LAL method. |
| Reconstitution: |
Centrifuge the vial briefly before opening to ensure that the contents settle at the bottom. Reconstitute the vial with sterile distilled water or an aqueous buffer containing 0.1% BSA to achieve a concentration of 0.1-1.0 mg/mL. Divide the resulting stock solution into working aliquots and store them at or below -20°C. For further dilutions, use appropriate buffered solutions. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Synonyms: |
Iroplact, Oncostatin-A |
| Background: |
Platelet Factor-4 (PF-4), or chemokine (C-X-C motif) ligand 4 (CXCL4), is a small cytokine within the CXC chemokine family. The human CXCL4 gene resides on chromosome 4. Released from the alpha-granules of activated platelets during aggregation, CXCL4 counteracts the anticoagulant effects of heparin and interacts with a splice variant of the CXCR3 receptor. It is chemotactic for neutrophils, fibroblasts, and monocytes. The recombinant human CXCL4 comprises 70 amino acids and binds heparin with high affinity. It shares approximately 60% amino acid sequence identity with its mouse counterpart. |
