| Source: |
Escherichia coli. |
| Molecular Weight: |
Approximately 24.8 kDa, a disulfide-linked homodimeric protein containing two 110 amino acid residues polypeptide (B chain). |
| AA Sequence: |
MSLGSLTIAE PAMIAECKTR TEVFEISRRL IDRTNANFLV WPPCVEVQRC SGCCNNRNVQ CRPTQVQLRP VQVRKIEIVR KKPIFKKATV TLEDHLACKC ETVAAARPVT |
| Purity: |
> 97% by SDS-PAGE and HPLC analyses. |
| Biological Activity: |
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 3 ng/mL, corresponding to a specific activity of > 3.3 × 105 IU/mg. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH7.4. |
| Endotoxin: |
Less than 1 EU/μg of rHuPDGF-BB as determined by LAL method. |
| Reconstitution: |
Centrifuge the vial briefly before opening to ensure that the contents settle at the bottom. Reconstitute the vial with sterile distilled water or an aqueous buffer containing 0.1% BSA to achieve a concentration of 0.1-1.0 mg/mL. Divide the resulting stock solution into working aliquots and store them at or below -20°C. For further dilutions, use appropriate buffered solutions. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Synonyms: |
Glioma-derived Growth Factor, GDGF, Osteosarcoma-derived Growth Factor, ODGF, PDGF-2, Platelet-derived Growth Factor Beta Polypeptide, Becaplermin |
| Background: |
Platelet-derived growth factor (PDGF), first discovered in animal studies by Lynch and his team in the late 1980s, exists in serum but is not found in plasma. It consists of a dimer linked by disulfide and made up of two peptide chains known as chain A and chain B. PDGF comes in three varieties - PDGF-AA, PDGF-BB and PDGF-AB. This growth factor plays a role in a variety of biological functions including hyperplasia, development of embryonic neurons, chemotaxis, and the development of respiratory tubule epithelial cells. Its functioning is mediated via two receptors, namely PDGFR-α and PDGFR-β. |
