| Source: |
Escherichia coli |
| Molecular Weight: |
Approximately 28.8 kDa, a disulfide-linked homodimeric protein containing two 126 amino acid residues polypeptide. But it migrates with an apparent molecular mass of 33.6 kDa in SDS-PAGE. |
| AA Sequence: |
MSIEEAVPAV CKTRTVIYEI PRSQVDPTSA NFLIWPPCVE VKRCTGCCNT SSVKCQPSRV HHRSVKVAKV EYVRKKPKLK EVQVRLEEHL ECACATTSLN PDYREEDTGR PRESGKKRKR KRLKPT |
| Purity: |
> 95% by SDS-PAGE. |
| Biological Activity: |
Measured in a cell proliferation assay using NR6R-3T3 mouse fibroblast cells. The ED50 for this effect is 50200 ng/mL in a fluorometric assay using the redox sensitive dye. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from a 0.2 µm filtered concentrated solution in 20 mM PB, 300 mM NaCl, pH 6.0. |
| Endotoxin: |
Less than 0.1 EU/µg of rHuPDGF-AA as determined by LAL method. |
| Reconstitution: |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
