| Source: |
Escherichia coli |
| Molecular Weight: |
Approximately 12.7 kDa, a single non-glycosylated polypeptide chain containing 111 amino acids. |
| AA Sequence: |
VHGIFMDRLA SKKLCADDEC VYTISLASAQ EDYNAPDCRF INVKKGQQIY VYSKLVKENG AGEFWAGSVY GDGQDEMGVV GYFPRNLVKE QRVYQEATKE VPTTDIDFFC E |
| Purity: |
> 97% by SDS-PAGE and HPLC analyses. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from a 0.2 µm filtered concentrated solution in 20 mM PB, pH7.4, 150 mM NaCl. |
| Endotoxin: |
Less than 1 EU/µg of rHuOTOR as determined by LAL method. |
| Reconstitution: |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Synonyms: |
Fibrocyte-derived Protein, Melanoma Inhibitory Activity-like Protein |
| Background: |
OTOR, also known as Otoraplin and MIAL, is a 15 kDa disulfide-bonded homodimer secreted via the Golgi apparatus. It belongs to the MIA/OTOR family, characterized by a Src homology-3 (SH3)-like domain. OTOR is predominantly expressed in the cochlea of the inner ear and plays a role in the early chondrogenesis of the otic capsule. This function is crucial for normal development of the inner ear and auditory function. OTOR shares significant sequence identity with orthologs in mice (90%), chicken (80%), and bullfrog (60%), as well as with the related human CDRAP/MIA protein (43%). |
