| Source: |
Escherichia coli |
| Molecular Weight: |
Approximately 22.3 kDa, a single non-glycosylated polypeptide chain containing 198 amino acids. |
| AA Sequence: |
LNRTGDPGPG PSIQKTYDLT RYLEHQLRSL AGTYLNYLGP PFNEPDFNPP RLGAETLPRA TVDLEVWRSL NDKLRLTQNY EAYSHLLCYL RGLNRQAATA ELRRSLAHFC TSLQGLLGSI AGVMAALGYP LPQPLPGTEP TWTPGPAHSD FLQKMDDFWL LKELQTWLWR SAKDFNRLKK KMQPPAAAVT LHLGAHGF |
| Purity: |
> 98% by SDS-PAGE and HPLC analyses. |
| Biological Activity: |
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 4 ng/mL, corresponding to a specific activity of > 2.5 × 105 IU/mg in the presence of human CNTF R alpha. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from a 0.2 μm filtered concentrated solution in 30% acetonitrile and 0.1% TFA. |
| Endotoxin: |
Less than 0.1 EU/μg of rHuNNT-1/BCSF-3 as determined by LAL method. |
| Reconstitution: |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Synonyms: |
Cardiotrophin-like cytokine factor 1, BSF-3 |
| Background: |
Novel neurotrophin-1 (NNT-1), also known as B-Cell Stimulating Factor-3 (BSF-3), is a recent addition to the IL-6 family of cytokines, which includes IL-6, CNTF, LIF, CT-1, IL-11, and OSM. These cytokines all utilize the gp130 receptor subunit, belonging to the type I cytokine receptor superfamily. Upon ligand binding, gp130 can form homodimers or heterodimers with receptors such as LIF receptor or OSM receptor beta, initiating cellular signaling and functional responses. For several members of this family, such as CNTF, IL-6, and IL-11, binding of the ligand to a specific alpha subunit receptor (CNTF R alpha, IL-6 R alpha, or IL-11 R alpha) is a prerequisite for subsequent gp130 homodimerization or heterodimerization. |
