| Source: |
Escherichia coli. |
| Molecular Weight: |
Approximately 46.3 kDa, non-disulfide-linked homodimer consisting of two 206 amino acid polypeptide chains. |
| AA Sequence: |
MQHYLHIRPA PSDNLPLVDL IEHPDPIFDP KEKDLNETLL RSLLGGHYDP GFMATSPPED RPGGGGGAAG GAEDLAELDQ LLRQRPSGAM PSEIKGLEFS EGLAQGKKQR LSKKLRRKLQ MWLWSQTFCP VLYAWNDLGS RFWPRYVKVG SCFSKRSCSV PEGMVCKPSK SVHLTVLRWR CQRRGGQRCG WIPIQYPIIS ECKCSC |
| Purity: |
> 95% by SDS-PAGE and HPLC analyses. |
| Biological Activity: |
Fully biologically active when compared to standard. The ED50 as determined by inhibiting BMP-4-induced alkaline phosphatase production of murine ATDC5 cells is less than 3.0 ng/mL, corresponding to a specific activity of > 3.3 × 105 IU/mg in the presence of 5 ng/mL rHuBMP-4. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from a 0.2 μm filtered concentrated solution in 30% acetonitrile, 0.1% TFA. |
| Endotoxin: |
Less than 1 EU/μg of rHuNoggin as determined by LAL method. |
| Reconstitution: |
Centrifuge the vial briefly before opening to ensure that the contents settle at the bottom. Reconstitute in 10 mM HCl to a concentration of 0.1-1.0 mg/mL. Divide the resulting stock solution into working aliquots and store them at or below -20°C. For further dilutions, use appropriate buffered solutions. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Background: |
Noggin, a protein encoded by the NOG gene, was first identified in the frog species Xenopus as a molecule capable of inducing the formation of a secondary axis in embryos. It functions by blocking TGF-β family ligands, preventing them from attaching to their receptors. Initially recognized as an antagonist to BMP-4, noggin has since been found to regulate the activity of other BMPs, including BMP-2, 7, 13, and 14. Its effects are wide-ranging, influencing both early development and later stages. Mouse studies lacking noggin indicate its involvement in various developmental processes, such as the merging of the neural tube and the formation of joints. Recent findings link mutations in evolutionarily conserved amino acid residues of noggin to conditions like proximal symphalangism (SYM1) and multiple synostoses syndrome (SYNS1). The amino acid sequence of mature human noggin is highly similar to that of other species, sharing 99% identity with mouse and rat, 98% with bovine, 97% with equine, and 89% with chicken noggin. |
