| Source: |
Escherichia coli. |
| Molecular Weight: |
Approximately 7.0 kDa, a single non-glycosylated polypeptide chain containing 61 amino acids. |
| AA Sequence: |
SHLVKCAEKE KTFCVNGGEC FMVKDLSNPS RYLCKCPNEF TGDRCQNYVM ASFYKAEELY Q |
| Purity: |
> 96% by SDS-PAGE and HPLC analyses. |
| Biological Activity: |
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using serum free human MCF-7 cells is less than 5 ng/mL, corresponding to a specific activity of > 2.0 × 105 U/mg. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from a 0.2 μm filtered solution in PBS, pH7.4. |
| Endotoxin: |
Less than 1 EU/μg of rHuNRG1-β2 as determined by LAL method. |
| Reconstitution: |
Centrifuge the vial briefly before opening to ensure that the contents settle at the bottom. Reconstitute the vial with sterile distilled water or an aqueous buffer containing 0.1% BSA to achieve a concentration of 0.1-1.0 mg/mL. Divide the resulting stock solution into working aliquots and store them at or below -20°C. For further dilutions, use appropriate buffered solutions. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Synonyms: |
Heregulin-beta1, HRG1 |
| Background: |
Neuregulin 1 is part of a family of structurally connected growth factors in the form of polypeptides. Produced via multiple alternative splicing processes, it has various isoforms which perform a broad spectrum of functions. These include heregulins (HRGs), glial growth factors (GGFs), and a sensory and motor neuron-derived factor (SMDF). Each one possesses an Ig and EGF-like domain, enabling them to bind to ErbB3 and ErbB4 receptor tyrosin kinases. Such binding causes the heterodimerization of ErbB3 and ErbB4 with ErbB2, prompting the activation of intrinsic kinase activity and consequent tyrosine phosphorylation. NRG1 isoforms play a role in prompting the growth and differentiation of various cells including epithelial, neuronal, and glial cells. |
