| Source: |
Escherichia coli |
| Molecular Weight: |
Approximately 9.6 kDa, a single non-glycosylated polypeptide chain containing 82 amino acids. |
| AA Sequence: |
VPIDIDKTKV QNIHPVESAK IEPPDTGLYY DEYLKQVIDV LETDKHFREK LQKADIEEIK SGRLSKELDL VSHHVRTKLD EL |
| Purity: |
> 95% by SDS-PAGE and HPLC analyses. |
| Biological Activity: |
Fully biologically active when compared to standard. The biological activity is tested by in vivo assay using healthy wild type male mice (C57BL/6J). |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH7.4. |
| Endotoxin: |
Less than 1 EU/µg of rHuNesfatin-1 as determined by LAL method. |
| Reconstitution: |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Synonyms: |
Nucleobindin 2 |
| Background: |
Nesfatin is a metabolic polypeptide derived from the N-terminal region of the precursor protein Nucleobindin2, which is encoded by the NUCB2 gene. Originally identified as a hypothalamic neuropeptide, Nesfatin is naturally occurring and found not only in the hypothalamus but also in other brain regions, pancreatic isletsβ-cells, gastric endocrine cells, and adipocytes. It plays a crucial role in appetite regulation and body fat metabolism. Elevated levels of nesfatin-1 in the brain suppress appetite, reduce hunger frequency, induce a feeling of fullness, and lead to decreased body fat and weight. Conversely, decreased levels of nesfatin-1 in the brain increase appetite, heighten hunger frequency, promote weight gain, and inhibit the feeling of satiety. |
