| Source: |
Escherichia coli. |
| Molecular Weight: |
Approximately 8.6 kDa, a single non-glycosylated polypeptide chain containing 75 amino acids. |
| AA Sequence: |
QPDALNVPST CCFTFSSKKI SLQRLKSYVI TTSRCPQKAV IFRTKLGKEI CADPKEKWVQ NYMKHLGRKA HTLKT |
| Purity: |
> 96% by SDS-PAGE and HPLC analyses. |
| Biological Activity: |
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human monocytes is in a concentration of 10-100 ng/mL. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH7.4, 130 mM NaCl. |
| Endotoxin: |
Less than 1 EU/μg of rHuMCP-4/CCL13 as determined by LAL method. |
| Reconstitution: |
Centrifuge the vial briefly before opening to ensure that the contents settle at the bottom. Reconstitute the vial with sterile distilled water or an aqueous buffer containing 0.1% BSA to achieve a concentration of 0.1-1.0 mg/mL. Divide the resulting stock solution into working aliquots and store them at or below -20°C. For further dilutions, use appropriate buffered solutions. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Synonyms: |
CK-beta-10, NCC-1, Small-inducible cytokine A13 |
| Background: |
Human CCL13, a member of the CC chemokine family, is encoded by the gene CCL13. CCL13 (also known as MCP-4) shares 56-61% sequence identity with MCP-1 (CCL2) and MCP-3 (CCL7), and is approximately 60% similar to Eotaxin (CCL11). CCL13 acts as a potent chemoattractant for monocytes and eosinophils, stimulating histamine release from basophils. In HEK-293 cells transfected with the receptors CCR2B and CCR3, CCL13 induces a calcium flux. This finding suggests that the functional receptors for CCL3 are identical to CCR2B and CCR3. |
