| Source: |
Escherichia coli. |
| Molecular Weight: |
Approximately 9.0 kDa, a single, non-glycosylated polypeptide chain containing 76 amino acids. |
| AA Sequence: |
QPVGINTSTT CCYRFINKKI PKQRLESYRR TTSSHCPREA VIFKTKLDKE ICADPTQKWV QDFMKHLDKK TQTPKL |
| Purity: |
> 97% by SDS-PAGE and HPLC analyses. |
| Biological Activity: |
Fully biologically active when compared to standard. The biologically active determined by a chemotaxis bioassay using human monocytes is in a concentration range of 10-100 ng/mL. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH7.4, 150 mM NaCl. |
| Endotoxin: |
Less than 1 EU/μg of rHuMCP-3/CCL7 as determined by LAL method. |
| Reconstitution: |
Centrifuge the vial briefly before opening to ensure that the contents settle at the bottom. Reconstitute the vial with sterile distilled water or an aqueous buffer containing 0.1% BSA to achieve a concentration of 0.1-1.0 mg/mL. Divide the resulting stock solution into working aliquots and store them at or below -20°C. For further dilutions, use appropriate buffered solutions. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Synonyms: |
NC28, Small-inducible cytokine A7 |
| Background: |
Also known as MCP-3, Human CCL7 is a member of the CC chemokine family. Discovered by IL-1-beta stimulated human MG-63 osteosarcoma cells, it is closely related to MCP-1 and MCP-2, sharing 71% and 58% amino acid sequence identity, respectively. CCL7 can direct monocytes and eosinophils but not neutrophils and also attracts activated NK cells. |
