| Source: |
Escherichia coli. |
| Molecular Weight: |
Approximately 8.9 kDa, a single non-glycosylated polypeptide chain containing 76 amino acids. |
| AA Sequence: |
QPDSVSIPIT CCFNVINRKI PIQRLESYTR ITNIQCPKEA VIFKTKRGKE VCADPKERWV RDSMKHLDQI FQNLKP |
| Purity: |
> 96% by SDS-PAGE and HPLC analyses. |
| Biological Activity: |
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood monocytes is in a concentration range of 10-100 ng/mL. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH7.4, 100 mM NaCl. |
| Endotoxin: |
Less than 1 EU/μg of rHuMCP-2/CCL8 as determined by LAL method. |
| Reconstitution: |
Centrifuge the vial briefly before opening to ensure that the contents settle at the bottom. Reconstitute the vial with sterile distilled water or an aqueous buffer containing 0.1% BSA to achieve a concentration of 0.1-1.0 mg/mL. Divide the resulting stock solution into working aliquots and store them at or below -20°C. For further dilutions, use appropriate buffered solutions. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Synonyms: |
HC14, Small-inducible cytokine A8 |
| Background: |
Human CCL8, also known as monocyte chemotactic protein 2 (MCP-2), is a member of the CC chemokine family. It is encoded by the CCL8 gene. MCP-2 is structurally similar to both MCP-1 (CCL2) and MCP-3 (CCL7). All three of these proteins were discovered in the IL-1-beta stimulated human MG-63 osteosarcoma cells. CCL8 shares 62% amino acid sequence similarity with MCP-1, and 58% with MCP-3. CCL8 acts as a chemo-attractant for monocytes, eosinophils, and neutrophils. In addition, it also draws activated NK cells towards the site of inflammation. |
