| Source: |
Escherichia coli. |
| Molecular Weight: |
Approximately 8.7 kDa, a single non-glycosylated polypeptide chain containing 76 amino acids. |
| AA Sequence: |
QPDAINAPVT CCYNFTNRKI SVQRLASYRR ITSSKCPKEA VIFKTIVAKE ICADPKQKWV QDSMDHLDKQ TQTPKT |
| Purity: |
> 96% by SDS-PAGE and HPLC analyses. |
| Biological Activity: |
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human monocytes is in a concentration range of 10-100 ng/mL. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH7.4, 100 mM NaCl. |
| Endotoxin: |
Less than 1 EU/μg of rHuMCP-1/CCL2 as determined by LAL method. |
| Reconstitution: |
Centrifuge the vial briefly before opening to ensure that the contents settle at the bottom. Reconstitute the vial with sterile distilled water or an aqueous buffer containing 0.1% BSA to achieve a concentration of 0.1-1.0 mg/mL. Divide the resulting stock solution into working aliquots and store them at or below -20°C. For further dilutions, use appropriate buffered solutions. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Synonyms: |
HC11, Monocyte chemoattractant protein 1, MCAF, MCP-1, Monocyte secretory protein JE, Small-inducible cytokine A2 |
| Background: |
CCL2, also called MCP-1 and small inducible cytokine A2, is part of the CC chemokine family. This small cytokine is a monomeric polypeptide with a molecular weight of roughly 13 kDa, anchored to endothelial cell plasma membranes by proteoglycan glycosaminoglycan side chains. CCL2, which binds to CCR2 and CCR4, has chemotactic properties for monocytes and basophils but not for neutrophils or eosinophils. It is implicated in diseases with monocytic infiltrates, such as psoriasis, rheumatoid arthritis, and atherosclerosis, possibly playing a role in monocyte recruitment to arterial walls during atherosclerosis. Mature human CCL2 shares 78-79% amino acid identity with canine, porcine, and equine CCL2, while the mouse and rat versions, extended by 49 amino acids, share only about 56% amino acid identity within the common region. |
