| Source: |
Escherichia coli |
| Molecular Weight: |
Approximately 36.9 kDa, a single non-glycosylated polypeptide chain containing 320 amino acids. |
| AA Sequence: |
MSYYHHHHHH DYDIPTTENL YFQGAMDPEF EPHSLRYNLT VLSWDGSVQS GFLAEVHLDG QPFLRYDRQK CRAKPQGQWA EDVLGNKTWD RETRDLTGNG KDLRMTLAHI KDQKEGLHSL QEIRVCEIHE DNSTRSSQHF YYDGELFLSQ NLETEEWTVP QSSRAQTLAM NVRNFLKEDA MKTKTHYHAM HADCLQELRR YLESGVVLRR TVPPMVNVTR SEASEGNITV TCRASSFYPR NIILTWRQDG VSLSHDTQQW GDVLPDGNGT YQTWVATRIC RGEEQRFTCY MEHSGNHSTH PVPSGKVLVL QSHKLGCFGG |
| Purity: |
> 95% by SDS-PAGE and HPLC analyses. |
| Biological Activity: |
Fully biologically active when compared to standard. The specific activity is determined by binding MICA antibody in ELISA. |
| Physical Appearance: |
Sterile colorless liquid. |
| Formulation: |
Supplied as a 0.2 μm filtered concentrated sterile solution in PBS, pH7.4, and 8 M Urea. |
| Endotoxin: |
Less than 1 EU/µg of rHuMIC-A, His as determined by LAL method. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Refer to lot specific COA for the Use by Date when stored at ≤ -20°C as supplied. |
| Background: |
MIC-A (MHC class I chain-related gene A) is a type I transmembrane protein. It is found on the cell surface of gastric epithelium, endothelial cells, and fibroblasts, and within the cytoplasm of keratinocytes and monocytes. MIC-A expression can also be induced by bacterial and viral infections. It shares 85% amino acid identity with MIC-B and is distantly related to classical MHC class I proteins. Unlike classical MHC class I molecules, MIC-A lacks β2 microglobulin and binds as a monomer to the activating receptor KLRK1/NKG2D, which is expressed on NK cells, NKT cells, γδ T cells, and CD8+ αβ T cells, owing to its three extracellular Ig-like domains. Recognition of MIC-A by NKG2D leads to activation of cytolytic activity and/or cytokine production by these effector cells. MIC-A recognition plays a crucial role in immune surveillance against tumors, viral infections, and autoimmune diseases. |
