| Source: |
Escherichia coli |
| Molecular Weight: |
Approximately 10.2 kDa, a single non-glycosylated polypeptide chain containing 92 amino acids. |
| AA Sequence: |
QFINDAETEL MMSKLPLENP VVLNSFHFAA DCCTSYISQS IPCSLMKSYF ETSSECSKPG VIFLTKKGRQ VCAKPSGPGV QDCMKKLKPY SI |
| Purity: |
> 97% by SDS-PAGE and HPLC analyses. |
| Biological Activity: |
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human T-lymphocytes is in a concentration range of 1.0-10 ng/mL. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH7.4, 100 mM NaCl. |
| Endotoxin: |
Less than 1 EU/μg of rHuMIP-5/CCL15 as determined by LAL method. |
| Reconstitution: |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Synonyms: |
C-C Motif Chemokine 15, LKN-1, Mrp-2b, NCC-3, Small-inducible Cytokine A15 |
| Background: |
Human CCL15 belongs to the CC chemokine family and shares 35% amino acid homology with human HCC1 (CCL14). CCL15 is predominantly expressed in the heart, skeletal muscle, and adrenal gland, with lower expression observed in the liver, small intestine, and colon, as well as in certain leukocytes and lung macrophages. It acts as a chemotactic agent for neutrophils, monocytes, and lymphocytes by binding to cell surface chemokine receptors such as CCR1 and CCR3. Various cleaved forms of CCL15 exhibit greater potency as chemoattractants compared to intact CCL15. |
