| Source: |
Escherichia coli |
| Molecular Weight: |
Approximately 11.2 kDa, a single non-glycosylated polypeptide chain containing 97 amino acids. |
| AA Sequence: |
QPKVPEWVNT PSTCCLKYYE KVLPRRLVVG YRKALNCHLP AIIFVTKRNR EVCTNPNDDW VQEYIKDPNL PLLPTRNLST VKIITAKNGQ PQLLNSQ |
| Purity: |
> 97% by SDS-PAGE and HPLC analyses. |
| Biological Activity: |
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human monocytes is in a concentration range of 10-100 ng/mL. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH7.4, 150 mM NaCl. |
| Endotoxin: |
Less than 1 EU/μg of rHuLEC/CCL16 as determined by LAL method. |
| Reconstitution: |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Synonyms: |
HCC-4, LCC-1, LMC, MTN-1, NCC-4 |
| Background: |
Human CCL16, also known as liver-expressed chemokine (LEC), monotactin-1 (MTN-1), and IL-10-inducible chemokine, is encoded by the CCL16 gene located on chromosome 17 in humans. This gene encodes a precursor protein of 120 amino acids, including a 23 amino acid signal peptide that is cleaved to yield a mature protein of 97 amino acids. CCL16 is primarily secreted by liver cells, thymus cells, and spleen cells, and it exhibits chemotactic activity towards lymphocytes and monocytes. It is distantly related to other CC chemokines, sharing less than 30% sequence identity with them. CCL16 expression is highly induced by IL-10, IFN-γ, and bacterial lipopolysaccharide in monocytes, and it signals through receptors CCR1, CCR2, CCR5, and CCR8. |
