| Source: |
Escherichia coli. |
| Molecular Weight: |
Approximately 19.1 kDa, a single, non-glycosylated polypeptide chain containing 169 amino acids. |
| AA Sequence: |
LGQDMVSPEA TNSSSSSFSS PSSAGRHVRS YNHLQGDVRW RKLFSFTKYF LKIEKNGKVS GTKKENCPYS ILEITSVEIG VVAVKAINSN YYLAMNKKGK LYGSKEFNND CKLKERIEEN GYNTYASFNW QHNGRQMYVA LNGKGAPRRG QKTRRKNTSA HFLPMVVHS |
| Purity: |
> 97% by SDS-PAGE and HPLC analyses. |
| Biological Activity: |
Fully biologically active when compared to standard. The ED50 as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 0.5 ng/mL, corresponding to a specific activity of > 2.0 × 106 IU/mg. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from a 0.2 µm filtered concentrated solution in 2 × PBS, pH7.4. |
| Endotoxin: |
Less than 1 EU/µg of rHuKGF-2/FGF-10 as determined by LAL method. |
| Reconstitution: |
Centrifuge the vial briefly before opening to ensure that the contents settle at the bottom. Reconstitute the vial with sterile distilled water or an aqueous buffer containing 0.1% BSA to achieve a concentration of 0.1-1.0 mg/mL. Divide the resulting stock solution into working aliquots and store them at or below -20°C. For further dilutions, use appropriate buffered solutions. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Background: |
Fibroblast growth factor 10, a part of the fibroblast growth factor (FGF) family, plays a crucial role in various biological processes that include embryonic development, cell growth, morphogenesis, tissue repair, and the growth and invasion of tumours. Like most other members of the FGF family, FGF-10 contains a heparin-binding domain and is key in regulating embryonic development, as well as cell proliferation and differentiation. FGF-10 also may play a part in wound healing and is necessary for normal branching morphogenesis. The recombinant human FGF-10 consists of 208 amino acids and shares 92% sequence identity with murine FGF-10 and 95% with rat FGF-10. FGF-10 defects can cause autosomal dominant aplasia of lacrimal and salivary glands and lacrimo-auriculo-dento-digital syndrome. |
