Cat. No.: | SPODRP01605 |
Size: | |
Quantity: |
|
Pricey: | Inquiry |
Source: | Escherichia coli. |
Molecular Weight: | Approximately 14.1 kDa, a single non-glycosylated polypeptide chain containing 126 amino acids. |
AA Sequence: | QGCPTLAGIL DINFLINKMQ EDPASKCHCS ANVTSCLCLG IPSDNCTRPC FSERLSQMTN TTMQTRYPLI FSRVKKSVEV LKNNKCPYFS CEQPCNQTTA GNALTFLKSL LEIFQKEKMR GMRGKI |
Purity: | > 95% by SDS-PAGE and HPLC analyses. |
Biological Activity: | Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human MO7e cells is less than 0.2 ng/mL, corresponding to a specific activity of > 5.0 × 106 IU/mg. |
Physical Appearance: | Sterile filtered white lyophilized (freeze-dried) powder. |
Formulation: | Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH7.4. |
Endotoxin: | Less than 1 EU/µg of rHuIL-9 as determined by LAL method. |
Reconstitution: | Centrifuge the vial briefly before opening to ensure that the contents settle at the bottom. Reconstitute the vial with sterile distilled water or an aqueous buffer containing 0.1% BSA to achieve a concentration of 0.1-1.0 mg/mL. Divide the resulting stock solution into working aliquots and store them at or below -20°C. For further dilutions, use appropriate buffered solutions. |
Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 month, 2 to 8°C under sterile conditions after reconstitution. 3 months, -20 to -70°C under sterile conditions after reconstitution. |
Synonyms: | Cytokine P40, T-cell Growth Factor P40 |
Background: | The IL9 gene is responsible for producing IL-9, which is secreted by T-cells, particularly CD4+ helper cells. Initially discovered in a T cell line transformed by a human T cell leukemia virus, IL-9 operates via its receptor, activating various STAT proteins and influencing numerous biological functions. It aids the proliferation of certain helper T-cells independent of IL-2 and IL-4. Human IL-9 shares more than half of its amino acid sequence with its murine counterpart and is implicated in asthma, playing a role in the development of bronchial sensitivity. |