| Source: |
Escherichia coli |
| Molecular Weight: |
Approximately 17.4 kDa, a single non-glycosylated polypeptide chain containing 152 amino acids. |
| Purity: |
> 97% by SDS-PAGE and HPLC analyses. |
| Biological Activity: |
Fully biologically active when compared to standard. rHuIL-7 stimulates proliferation of PHA-activated human peripheral blood mononuclear cell (PBMC). The specific activity of Recombinant Human IL-7 is > 1.0 × 108 Units/mg, which is calibrated against human IL-7 WHO standard. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from a 0.2 µm filtered concentrated solution in 20 mM PB, 150 mM NaCl, pH7.4. |
| Endotoxin: |
Less than 1 EU/µg of rHuIL-7 as determined by LAL method. |
| Reconstitution: |
Prior to opening, it is recommended to centrifuge the vial briefly to bring the contents down the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. If animal-origin-free condition is expected in your product, then sterile distilled water is recommended. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
A minimum of 12 months from date of receipt, when stored at ≤ -20°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Synonyms: |
LP-1, pre-B cell factor |
| Background: |
Interleukin-7 (IL-7) originates from the IL7 gene and is released by stromal cells found in the red marrow and thymus. Upon secretion, it interacts with the IL-7 receptor, composed of the IL-7 receptor alpha and IL-2 receptor gamma chain heterodimer. IL-7 plays a pivotal role in prompting hematopoietic stem cells to differentiate into lymphoid progenitor cells, and it also fosters the proliferation of B cells, T cells, and NK cells. With about 65% amino acid sequence similarity between murine and human IL-7, both variants demonstrate activity across species. IL-7's potential as an immunotherapy has undergone extensive exploration in numerous human clinical trials targeting various malignancies and HIV infection. |
| Sequence: |
DCDIEGKDGK QYESVLMVSI DQLLDSMKEI GSNCLNNEFN FFKRHICDAN KEGMFLFRAA RKLRQFLKMN STGDFDLHLL KVSEGTTILL NCTGQVKGRK PAALGEAQPT KSLEENKSLK EQKKLNDLCF LKRLLQEIKT CWNKILMGTK EH |
