| Source: |
Spodoptera frugiperda, Sf 21 (baculovirus) |
| Molecular Weight: |
Apparent molecular mass of 45-50 kDa in SDS-PAGE under reducing conditions, a single glycosylated polypeptide protein consisting of 339 amino acids. |
| AA Sequence: |
Leu20-Asp358; Accession # P08887.1 |
| Purity: |
> 97% by SDS-PAGE analyses. |
| Biological Activity: |
Measured by its ability to enhance the IL-6 activity on M1 mouse myeloid leukemia cells. The ED50 for this effect is 5-15 ng/mL. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from 0.2 µm filtered concentrated solution in PBS. |
| Endotoxin: |
Less than 1 EU/µg of rHuIL-6 Rα as determined by LAL method. |
| Reconstitution: |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile PBS or aqueous buffer to a concentration of 0.1 mg/mL. Further dilutions should be made in appropriately buffered solutions. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
