| Source: |
Escherichia coli. |
| Molecular Weight: |
Approximately 17.0 kDa, a single non-glycosylated polypeptide chain containing 152 amino acids. |
| AA Sequence: |
SMCKPITGTI NDLNQQVWTL QGQNLVAVPR SDSVTPVTVA VITCKYPEAL EQGRGDPIYL GIQNPEMCLY CEKVGEQPTL QLKEQKIMDL YGQPEPVKPF LFYRAKTGRT STLESVAFPD WFIASSKRDQ PIILTSELGK SYNTAFELNI ND |
| Purity: |
> 95% by SDS-PAGE and HPLC analyses. |
| Biological Activity: |
Fully biologically active when compared to standard. The ED50 as determined by its ability to induce IL-8 secretion by human preadipocytes is less than 10 ng/mL, corresponding to a specific activity of > 1 × 105 IU/mg. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH7.4. |
| Endotoxin: |
Less than 1 EU/μg of rHuIL-36γ, 152a.a. as determined by LAL method. |
| Reconstitution: |
Centrifuge the vial briefly before opening to ensure that the contents settle at the bottom. Reconstitute the vial with sterile distilled water or an aqueous buffer containing 0.1% BSA to achieve a concentration of 0.1-1.0 mg/mL. Divide the resulting stock solution into working aliquots and store them at or below -20°C. For further dilutions, use appropriate buffered solutions. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Synonyms: |
IL-1RP2, IL-1 epsilon, IL-1F9, IL-1H1 |
| Background: |
Interleukin-36 (IL-36), a pro-inflammatory cytokine, significantly influences the pathophysiology of various diseases. IL-36α, IL-36β, and IL-36γ, part of the IL-1 family, activate signaling through IL-1Rrp2 and IL-1RAcP receptors. IL-36γ, when introduced into 293-T cells, may operate within a signaling system akin to IL-1A, IL-1B receptor agonist, and IL-1R1, and can also trigger NF-kappa B activation via the orphan IL-1-receptor-related protein 2. Human IL-36γ, a 152 amino acid protein, exhibits 58%, 59%, 68%, and 69% sequence identity with mouse, rat, bovine, and equine IL-36γ, respectively, and shares 23-57% identity with other family members. |
