| Source: |
Escherichia coli. |
| Molecular Weight: |
Approximately 17.7 kDa, a single non-glycosylated polypeptide chain containing 158 amino acids. |
| AA Sequence: |
MEKALKIDTP QQGSIQDINH RVWVLQDQTL IAVPRKDRMS PVTIALISCR HVETLEKDRG NPIYLGLNGL NLCLMCAKVG DQPTLQLKEK DIMDLYNQPE PVKSFLFYHS QSGRNSTFES VAFPGWFIAV SSEGGCPLIL TQELGKANTT DFGLTMLF |
| Purity: |
> 95% by SDS-PAGE and HPLC analyses. |
| Biological Activity: |
Fully biologically active when compared to standard. The specific activity determined by its ability in a functional ELISA. Immobilized rHuIL-36α at 1 µg/mL can bind recombinant human IL-1 Rrp2 Fc Chimera with a range of 0.15-5 µg/mL. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from a 0.2 μm filtered concentrated solution in 2 × PBS, pH7.4. |
| Endotoxin: |
Less than 1 EU/μg of rHuIL-36α, 158a.a. as determined by LAL method. |
| Reconstitution: |
Centrifuge the vial briefly before opening to ensure that the contents settle at the bottom. Reconstitute the vial with sterile distilled water or an aqueous buffer containing 0.1% BSA to achieve a concentration of 0.1-1.0 mg/mL. Divide the resulting stock solution into working aliquots and store them at or below -20°C. For further dilutions, use appropriate buffered solutions. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Synonyms: |
FIL1 epsilon, IL-1 epsilon, IL-1F6, IL-1H1 |
| Background: |
Interleukin-36 (IL-36) is a pro-inflammatory cytokine which has a significant impact within the pathophysiology of multiple diseases. The members of the IL-1 family namely IL-36α, IL-36β, and IL-36γ (which were previously referred to as IL-1F6, IL-1F8, and IL -1F9), transmit signals via IL-1 receptor family members IL-1Rrp2 (IL-1RL2) and IL-1RAcP. IL-36α has shown a large presence in skin and lymphoid tissues, and also in fetal brain, trachea, stomach, and intestine, according to studies. Interestingly, IL-36α is the sole new IL-1 family member that is expressed on T-cells. The recombinant human interleukin-36 alpha, existing as a single non-glycosylated polypeptide with 158 amino acids, shares a 30% a.a. identity with IL-1ra, and sequences identical by 27%, 31%, 36%, 46%, 57%, and 28% to IL-1β, IL-36Ra/IL-1F5, IL-37/IL-1F7 , IL-36β/IL-1F8, IL-36γ/IL-1F9 and IL-1F10. |
