| Source: |
Escherichia coli. |
| Molecular Weight: |
Approximately 17.9 kDa, a single non-glycosylated polypeptide chain containing 159 amino acids. |
| AA Sequence: |
SITGISPITE YLASLSTYND QSITFALEDE SYEIYVEDLK KDEKKDKVLL SYYESQHPSN ESGDGVDGKM LMVTLSPTKD FWLHANNKEH SVELHKCEKP LPDQAFFVLH NMHSNCVSFE CKTDPGVFIG VKDNHLALIK VDSSENLCTE NILFKLSET |
| Purity: |
> 97% by SDS-PAGE and HPLC analyses. |
| Biological Activity: |
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine D10S cells is less than 0.05 ng/mL, corresponding to a specific activity of > 2.0 × 107 IU/mg. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from a 0.2μm filtered concentrated solution in 20 mM PB, 150 mM NaCl, 1mM EDTA, pH7.4. |
| Endotoxin: |
Less than 1 EU/µg of rHuIL-33 as determined by LAL method. |
| Reconstitution: |
Centrifuge the vial briefly before opening to ensure that the contents settle at the bottom. Reconstitute the vial with sterile distilled water or an aqueous buffer containing 0.1% BSA to achieve a concentration of 0.1-1.0 mg/mL. Divide the resulting stock solution into working aliquots and store them at or below -20°C. For further dilutions, use appropriate buffered solutions. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Synonyms: |
IL-1F11, NF-HEV, DVS 27 |
| Background: |
The IL-33 gene, located on chromosome 9 in humans, encodes a 30 kDa inflammatory protein that is part of the IL-1 superfamily and shares less than 20% sequence identity with its family members. IL-33 is highly secreted by high endothelial venules found in tonsils, Peyer's patches, and mesenteric lymph nodes, but not in the placenta. Its expression is increased in arterial smooth muscle, skin fibroblasts, and skin cells following stimulation by IL1α or IL1β. IL-33 exerts its effects by binding to the IL1RL1/ST2 receptor, leading to the recruitment of MYD88, IRAK1, IRAK4, and TRAF6, and subsequent activation of MAPK pathways. IL-33 mature protein has 52-58% a.a. sequence identity with mouse and rat IL-33. |
