| Source: |
Escherichia coli. |
| Molecular Weight: |
Approximately 15.8 kDa, a single non-glycosylated polypeptide chain containing 141 amino acids. |
| AA Sequence: |
SHTLPVRLLR PSDDVQKIVE ELQSLSKMLL KDVEEEKGVL VSQNYTLPCL SPDAQPPNNI HSPAIRAYLK TIRQLDNKSV IDEIIEHLDK LIFQDAPETN ISVPTDTHEC KRFILTISQQ FSECMDLALK SLTSGAQQAT T |
| Purity: |
> 97% by SDS-PAGE and HPLC analyses. |
| Biological Activity: |
Fully biologically active when compared to standard. The specific activity is determined by inducing STAT3 activation using human U-87 MG cells. 5 ng/mL of rHuIL-31 can effectively induce STAT3 activation. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH7.4. |
| Endotoxin: |
Less than 1 EU/µg of rHuIL-31 as determined by LAL method. |
| Reconstitution: |
Centrifuge the vial briefly before opening to ensure that the contents settle at the bottom. Reconstitute the vial with sterile distilled water or an aqueous buffer containing 0.1% BSA to achieve a concentration of 0.1-1.0 mg/mL. Divide the resulting stock solution into working aliquots and store them at or below -20°C. For further dilutions, use appropriate buffered solutions. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Background: |
The IL-31 gene in humans is situated on chromosome 12. It is responsible for producing the IL-31 protein at minimal levels within type 2 helper T cells, which are present in various tissues including the testis, bone marrow, skeletal muscle, kidney, colon, thymus, small intestine, and trachea. This protein is structurally and functionally akin to IL-6, Oncostatin M, LIF, and Cardiotrophin-1. IL-31 communicates signals via the IL-31 receptor A and Oncostatin M receptor subunits, potentially playing a role in skin immunity by activating STAT3 through these receptors. It is involved in regulating immune responses that are linked to skin health and inflammatory skin disorders. The human IL-31 protein shares a 24% amino acid sequence identity with its mouse counterpart in the mature form. |
