| Source: |
Insect Cell |
| Molecular Weight: |
Approximately 60.3 kDa on SDS-PAGE under reducing conditions, containing 529 amino acids. |
| AA Sequence: |
AGMGTKEDPN PPITNLRMKA KAQQLTWDLN RNVTDIECVK DADYSMPAVN NSYCQFGAIS LCEVTNYTVR VANPPFSTWI LFPENSGKPW AGAENLTCWI HDVDFLSCSW AVGPGAPADV QYDLYLNVAN RRQQYECLHY KTDAQGTRIG CRFDDISRLS SGSQSSHILV RGRSAAFGIP CTDKFVVFSQ IEILTPPNMT AKCNKTHSFM HWKMRSHFNR KFRYELQIQK RMQPVITEQV RDRTSFQLLN PGTYTVQIRA RERVYEFLSA WSTPQRFECD QEEGANTRAW RIEGRMDEPK SSDKTHTCPP CPAPEFEGAP SVFLFPPKPK DTLMISRTPE VTCVVVDVSH EDPEVKFNWY VDGVEVHNAK TKPREEQYNS TYRVVSVLTV LHQDWLNGKE YKCKVSNKAL PTPIEKTISK AKGQPREPQV YTLPPSRDEL TKNQVSLTCL VKGFYPSDIA VEWESNGQPE NNYKTTPPVL DSDGSFFLYS KLTVDKSRWQ QGNVFSCSVM HEALHNHYTQ KSLSLSPGK |
| Purity: |
> 95% by SDS-PAGE analyses. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH7.0, with 0.02% Tween-20. |
| Endotoxin: |
Less than 0.01 EU/μg of rHuIL-3 Rα/CD123-Fc as determined by LAL method. |
| Reconstitution: |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Background: |
The interleukin-3 receptor (IL-3 R) is a heterodimer consisting of a 70 kDa IL-3 R alpha subunit (IL-3 Rα or CD123) and a 120-140 kDa IL-3 R beta subunit (IL-3 Rβ or CD131). IL-3Rα, a glycoprotein part of the hematopoietic receptor superfamily, binds IL-3 with low affinity. However, in the presence of IL-3 Rβ, IL-3 Rα binds IL-3 with much higher affinity. Recent studies show that CD123, the IL-3 Rα, is highly expressed in leukemic stem cells (LSCs) but not in normal hematopoietic stem cells (HSCs). This expression is linked to treatment response, minimal residual disease (MRD) detection, and prognosis. Additionally, CD123 is a crucial marker for identifying and targeting LSCs in refractory or relapsed leukemia. |
