| Source: |
Escherichia coli |
| Molecular Weight: |
Approximately 15.4 kDa, a single non-glycosylated polypeptide chain containing 133 amino acids. |
| Purity: |
> 98% by SDS-PAGE and HPLC analyses. |
| Biological Activity: |
Fully biologically active when compared to standard. Measured by its ability to enhance IFN-gamma secretion in NK-92 human natural killer lymphoma cells. The ED50 for this effect is ≤ 2 ng/mL. The specific activity of recombinant human IL-21 > 5.0 × 105 U/mg. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH7.0. |
| Endotoxin: |
Less than 0.01 EU/µg of rHuIL-21 GMP as determined by LAL method. |
| Reconstitution: |
Centrifuge the vial briefly before opening to ensure that the contents settle at the bottom. Reconstitute the vial with sterile distilled water or an aqueous buffer containing 0.1% BSA to achieve a concentration of 0.1-1.0 mg/mL. Divide the resulting stock solution into working aliquots and store them at or below -20°C. For further dilutions, use appropriate buffered solutions. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Background: |
Human interleukin-21 (IL-21), which is encoded by the IL21 gene found on chromosome 4, is a versatile cytokine important in the immune system's response to antigenic stimuli. It is primarily produced by CD4+ T cells and plays a crucial role in regulating different immune cells such as cytotoxic T cells and natural killer cells. Notably, IL-21 can trigger cell division or proliferation in target cells.
IL-21 works by binding to the interleukin-21 receptor (IL-21R), which has a gamma chain similar to that in other cytokine receptors like IL-2, IL-4, IL-7, IL-9, and IL-15. The interaction between IL-21 and IL-21R initiates a series of reactions, activating the tyrosine kinases JAK1 and JAK3, which in turn activates the transcription factors STAT1 and STAT3. IL-21 is significantly associated with various clinical conditions, including cancer immunotherapy, viral infections, and allergic reactions. |
| Shipping: |
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Sequence: |
QGQDRHMIRM RQLIDIVDQL KNYVNDLVPE FLPAPEDVET NCEWSAFSCF QKAQLKSANT GNNERIINVS IKKLKRKPPS TNAGRRQKHR LTCPSCDSYE KKPPKEFLER FKSLLQKMIH QHLSSRTHGS EDS |
| Sterility: |
Negative. |
| Mycoplasma: |
Negative. |
| Host Cell Protein: |
Less than 0.05% when tested by ELISA. |
| Quality Statement: |
The manufacturing and testing of these products comply with ICH Q7 guidelines. |
| Host Cell DNA: |
Less than 20 ng/mg when tested by qPCR. |
| In Vitro Virus Assay: |
Negative. |
