| Source: |
Escherichia coli. |
| Molecular Weight: |
Approximately 15.4 kDa, a single non-glycosylated polypeptide chain containing 133 amino acids. |
| AA Sequence: |
APTSSSTKKT QLQLEHLLLD LQMILNGINN YKNPKLTRML TFKFYMPKKA TELKHLQCLE EELKPLEEVL NLAQSKNFHL RPRDLISNIN VIVLELKGSE TTFMCEYADE TATIVEFLNR WITFCQSIIS TLT |
| Purity: |
> 97% by SDS-PAGE and HPLC analyses. |
| Biological Activity: |
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine CTLL-2 cells is less than 0.1 ng/mL, corresponding to a specific activity of > 1.0 × 107 IU/mg. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH3.5. |
| Endotoxin: |
Less than 1.0 EU/µg of rHuIL-2 as determined by LAL method. |
| Reconstitution: |
Centrifuge the vial briefly before opening to ensure that the contents settle at the bottom. Reconstitute in sterile distilled H2O to a concentration of 0.1-1.0 mg/mL. Divide the resulting stock solution into working aliquots and store them at or below -20°C. For further dilutions, use appropriate buffered solutions. Do not reconstitute in cell culture media directly. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Synonyms: |
IL2, T-cell Growth Factor, TCGF, Aldesleukin |
| Background: |
Interleukin-2 (IL-2) is a powerful immunoregulatory lymphokine produced by T-cells in response to antigenic or mitogenic stimulation. IL-2/IL-2R signaling is required for T-cell proliferation and other fundamental functions crucial for immune response. The receptor of this cytokine (IL-2R) is a heterotrimeric protein complex whose gamma chain is also shared by IL-4, IL-7, IL-9, IL-15 and IL-21 receptors. IL-2 provides a stimulatory signal, which allows for the flexible licensing of T cells in the process of shaping and establishing tolerance and immunity. IL-2-sensitive T cells differentiate into T regulatory cells and are essential for the maintenance and regulation of the immune system. Also, IL-2 plays critical roles in activation-induced cell death (AICD), a process vital for the maintenance of regular homeostasis of the immune system. More recently, IL-2 is used to treat cancer and rare autoimmune disorders, due its crucial role in the growth and proliferation of T cells. |
