| Source: |
Escherichia coli. |
| Molecular Weight: |
Approximately 31.0 kDa, a disulfide-linked homodimer of two 132 amino acid polypeptide chains. |
| AA Sequence: |
GITIPRNPGC PNSEDKNFPR TVMVNLNIHN RNTNTNPKRS SDYYNRSTSP WNLHRNEDPE RYPSVIWEAK CRHLGCINAD GNVDYHMNSV PIQQEILVLR REPPHCPNSF RLEKILVSVG CTCVTPIVHH VA |
| Purity: |
> 95% by SDS-PAGE and HPLC analyses. |
| Biological Activity: |
Fully biologically active when compared to standard. The ED50 as determined by inducing IL-6 secretion of murine NIH/3T3 cells is less than 7.5 ng/mL, corresponding to a specific activity of > 1.3 × 105 IU/mg. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH7.4. |
| Endotoxin: |
Less than 1 EU/µg of rHuIL-17 as determined by LAL method. |
| Reconstitution: |
Centrifuge the vial briefly before opening to ensure that the contents settle at the bottom. Reconstitute in 4 mM HCl to a concentration of 0.1-1.0 mg/mL. Divide the resulting stock solution into working aliquots and store them at or below -20°C. For further dilutions, use appropriate buffered solutions. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Synonyms: |
Cytotoxic T-lymphocyte-associated antigen 8, CLTA-8
, IL-17A |
| Background: |
The IL17A gene, situated on chromosome 6, encodes Human Interleukin-17A (IL-17A), a member of the IL-17 cytokine family which includes IL-17A to IL-17F. These cytokines share a structural motif with four conserved cysteine residues essential for their spatial configuration, yet they are distinct from other cytokines in their sequence. IL-17A, produced by T cells, is structurally similar to a herpesvirus Saimiri protein and forms disulfide-linked homodimers. It shares 63% of its amino acid sequence with its murine counterpart and is known to prompt stromal cells to release both proinflammatory and hematopoietic cytokines when secreted by activated CD4+ T cells. |
