| Source: |
Escherichia coli. |
| Molecular Weight: |
Approximately 12.9 kDa, a single non-glycosylated polypeptide chain containing 114 amino acids. |
| AA Sequence: |
NWVNVISDLK KIEDLIQSMH IDATLYTESD VHPSCKVTAM KCFLLELQVI SLESGDASIH DTVENLIILA NNSLSSNGNV TESGCKECEE LEEKNIKEFL QSFVHIVQMF INTS |
| Purity: |
> 97% by SDS-PAGE and HPLC analyses. |
| Biological Activity: |
Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. The ED50
for this effect is 0.300-2.60 ng/mL. The specific activity of recombinant human IL-15 is ≥1.50 ×108units/mg, which is calibrated against the human IL-15 reference standard (NIBSC code: 95/554). |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH7.4. |
| Endotoxin: |
Less than 1 EU/µg of rHuIL-15 as determined by LAL method. |
| Reconstitution: |
Centrifuge the vial briefly before opening to ensure that the contents settle at the bottom. Reconstitute the vial with sterile distilled water or an aqueous buffer containing 0.1% BSA to achieve a concentration of 0.1-1.0 mg/mL. Divide the resulting stock solution into working aliquots and store them at or below -20°C. For further dilutions, use appropriate buffered solutions. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Background: |
Human Interleukin-15 (IL-15) is a product of the IL15 gene located on chromosome 4. The human version of this protein shares roughly 97% sequence identity with the simian version and roughly 73% with the murine version, and both the human and simian versions have been found to be active on murine cells. This protein, secreted in particular by mononuclear phagocytes like macrophages after a viral infection, plays multiple biological roles. Its primary functions include stimulating and maintaining cellular immune responses, and it is particularly instrumental in regulating the activation and proliferation of T cells and natural killer (NK) cells. Furthermore, IL-15 has many biological properties in common with IL-2, including the ability to stimulate T, B and NK cells. IL-15 signals through a complex composed of the beta chain of the IL-2/IL-15 receptor, and even though it lacks sequence homology with IL-2, it has been found to both bind and signal using both the beta and gamma chains of the IL-2 receptor. Also, an IL-15 specific binding protein has been independently cloned from a mouse T cell clone. |
