| Source: |
Spodoptera frugiperda, Sf 21 (baculovirus) |
| Molecular Weight: |
Apparent molecular mass of 60 kDa in SDS-PAGE under non-reducing conditions, 41 kDa and 29 kDa under reducing conditions, a disulfide-linked heterodimer consisting of the 306 amino acid rHuIL-12 p40 and the 197 amino acid rHuIL-12 p35 subunits. |
| AA Sequence: |
HuIL-12 p40: Ile23 - Ser328; Accession # P29460HuIL-12 p35: Arg23 - Ser219; Accession # P29459 |
| Purity: |
> 97% by SDS-PAGE. |
| Biological Activity: |
Measured in a cell proliferation assay using PHA-stimulated human T lymphoblasts. The ED50 for this effect is 0.01-0.05 ng/mL. The specific activity of rHuIL-12 is approximately 1.1 × 104 units/μg, which is calibrated against rHuIL-12 WHO standard. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH7.2. |
| Endotoxin: |
Less than 1.0 EU/µg of rHuIL-12 as determined by LAL method. |
| Reconstitution: |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile PBS to a concentration of 0.1 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriately buffered solutions. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Synonyms: |
Natural killer cell stimulatory factor chain 2, NKSF2, Cytotoxic lymphocyte maturation factor 40 kDa subunit, CLMF p40 |
| Background: |
Interleukin-12 (IL-12) is a heterodimeric cytokine comprising disulfide-linked p35 and p40 subunits. It is secreted by various immune cells, including monocytes, macrophages, dendritic cells, neutrophils, Langerhans cells, keratinocytes, microglia, and peripheral B cells. In humans, the p40 subunit can also pair with a p19 subunit to form IL-23. IL-12 signals through a receptor complex composed of IL-12 R beta 1 and IL-12 R beta 2. It stimulates Th1 immune responses by inducing IFN-gamma production from NK cells, T cells, and macrophages, and by enhancing the cytotoxic functions of NK cells and T cells. Additionally, IL-12 works synergistically with IL-18 to develop Th1 memory cells. |
