| Source: |
Escherichia coli |
| Molecular Weight: |
Approximately 18.6 kDa, a single non-glycosylated polypeptide chain containing 160 amino acids. |
| Purity: |
> 95% by SDS-PAGE and HPLC analyses. |
| Biological Activity: |
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine MC/9-2 cells is less than 1 ng/mL, corresponding to a specific activity of > 1.0 × 106 U/mg. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from a 0.2 µm filtered concentrated solution in PBS, 0.02% Tween-20, pH6.0. |
| Endotoxin: |
Less than 0.01 EU/µg of rHuIL-10 GMP as determined by LAL method. |
| Reconstitution: |
Centrifuge the vial briefly before opening to ensure that the contents settle at the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. If animal-origin-free condition is expected in your product, then sterile distilled water is recommended. Divide the resulting stock solution into working aliquots and store them at or below -20°C. For further dilutions, use appropriate buffered solutions. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Background: |
Known as the cytokine synthesis inhibitory factor, IL-10 is the founding member of its cytokine family, which includes IL-19 through IL-26. It is produced by various activated blood-forming cells, liver cells, skin cells, and cells in the placenta. Human IL-10 is effective on mouse cells, but the reverse is not true. Comprising 178 amino acids, IL-10 forms a 36 kDa dimer that interacts with IL-10 receptor chains, triggering a chain reaction involving JAK1, TYK2, and STAT3. IL-10Rβ is essential for this process, although it does not directly bind IL-10. This molecule is vital for managing viral, allergic, and autoimmune conditions, enhancing phagocytosis and Th2 responses while inhibiting antigen presentation and Th1 inflammatory reactions. |
| Shipping: |
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Sequence: |
SPGQGTQSEN SCTHFPGNLP NMLRDLRDAF SRVKTFFQMK DQLDNLLLKE SLLEDFKGYL GCQALSEMIQ FYLEEVMPQA ENQDPDIKAH VNSLGENLKT LRLRLRRCHR FLPCENKSKA VEQVKNAFNK LQEKGIYKAM SEFDIFINYI EAYMTMKIRN |
| Host Cell Protein: |
Less than 0.10% when tested by ELISA. |
| Quality Statement: |
The manufacturing and testing of these products comply with ICH Q7 guidelines. |
