| Source: |
Escherichia coli. |
| Molecular Weight: |
Approximately 18.0 kDa, a single non-glycosylated polypeptide chain containing 159 amino acids. |
| AA Sequence: |
SAPFSFLSNV KYNFMRIIKY EFILNDALNQ SIIRANDQYL TAAALHNLDE AVKFDMGAYK SSKDDAKITV ILRISKTQLY VTAQDEDQPV LLKEMPEIPK TITGSETNLL FFWETHGTKN YFTSVAHPNL FIATKQDYWV CLAGGPPSIT DFQILENQA |
| Purity: |
> 97% by SDS-PAGE and HPLC analyses. |
| Biological Activity: |
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine D10S cells is less than 1.0 pg/mL, corresponding to a specific activity of > 1.0 × 109 IU/mg. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH7.4. |
| Endotoxin: |
Less than 1.0 EU/µg of rHuIL-1α as determined by LAL method. |
| Reconstitution: |
Centrifuge the vial briefly before opening to ensure that the contents settle at the bottom. Reconstitute the vial with sterile distilled water or an aqueous buffer containing 0.1% BSA to achieve a concentration of 0.1-1.0 mg/mL. Divide the resulting stock solution into working aliquots and store them at or below -20°C. For further dilutions, use appropriate buffered solutions. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Synonyms: |
Lymphocyte-activating factor, LAF, Leukocyte Endogenous Mediator, LEM, Endogenous Pyrogen, EP, Mononuclear Cell Factor, MCF , Hematopoietin-1 |
| Background: |
Interleukin-1 alpha (IL-1α), a non-secreted proinflammatory cytokine, is primarily generated by activated macrophages, along with neutrophils, epithelial cells, and endothelial cells. Possessing metabolic, physiological, and haematopoietic functions, it is central in controlling immune responses. Both IL-1α and IL-1β bind to the identical receptor, and they share comparable biological qualities. The mature IL-1α amino acid sequence has a 60% to 70% degree of conservation amongst different species. Moreover, human IL-1 has demonstrated biological activity on murine cell lines. Recently, IL-1α has found novel application in cosmetic and dermatological formulations. It significantly aids in enhancing the derma architecture. |
