| Source: |
Escherichia coli. |
| Molecular Weight: |
Approximately 16.9 kDa, a single non-glycosylated polypeptide chain containing 144 amino acids. |
| AA Sequence: |
MQDPYVKEAE NLKKYFNAGH SDVADNGTLF LGILKNWKEE SDRKIMQSQI VSFYFKLFKN FKDDQSIQKS VETIKEDMNV KFFNSNKKKR DDFEKLTNYS VTDLNVQRKA IHELIQVMAE LSPAAKTGKR KRSQMLFRGR RASQ |
| Purity: |
> 98% by SDS-PAGE and HPLC analyses. |
| Biological Activity: |
Fully biologically active when compared to standard. The ED50 as measured in anti-viral assays using human HeLa cells infected with encephalomyocarditis (EMC) virus is 0.15-0.80 ng/mL. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH7.4. |
| Endotoxin: |
Less than 1 EU/µg of rHuIFN-γ as determined by LAL method. |
| Reconstitution: |
Centrifuge the vial briefly before opening to ensure that the contents settle at the bottom. Reconstitute the vial with sterile distilled water or an aqueous buffer containing 0.1% BSA to achieve a concentration of 0.1-1.0 mg/mL. Divide the resulting stock solution into working aliquots and store them at or below -20°C. For further dilutions, use appropriate buffered solutions. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Background: |
Interferon-gamma (IFN-γ), also referred to as Type II interferon or immune interferon, is primarily produced by T-lymphocytes and natural killer cells. This cytokine has no significant genetic resemblance to IFN-β or the varying IFN-α family proteins. The mature IFN-γ exists as non-covalently linked homodimers. It's crucial to note that human IFN-γ is particularly species-specific, demonstrating biological activity only in human and primate cells. Originally, IFN-γ was identified due to its antiviral properties. However, the protein also displays antiproliferative, immunoregulatory and proinflammatory functions and thus, plays a significant role in the host defense mechanisms. IFN-γ incites the production of several other cytokines, enhances the expression of class I and II MHC antigens, Fc receptors, and leukocyte adhesion molecules. Furthermore, it modulates macrophage functional factors, impacts isotype switching, encourages the secretion of immunoglobulins by B cells, and promotes TH1 cell differentiation and expansion. |
