| Source: |
Yeast |
| Molecular Weight: |
Approximately 19.4 kDa, a single polypeptide chain containing 166 amino acids. |
| AA Sequence: |
CDLPQTHSLG NRRALILLAQ MGRISHFSCL KDRHDFGFPE EEFDGHQFQK AQAISVLHEM IQQTFNLFST EDSSAAWEQS LLEKFSTELY QQLNDLEACV IQEVGVEETP LMNEDSILAV RKYFQRITLY LTEKKYSPCA WEVVRAEIMR SLSFSTNLQK RLRRKD |
| Purity: |
> 95% by SDS-PAGE analyses. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from a 0.2 μm filtered solution in PBS, pH7.0. |
| Endotoxin: |
Less than 1 EU/μg of rHuIFN-α4a, Yeast as determined by LAL method. |
| Reconstitution: |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Background: |
Interferon-alpha (IFN-alpha), also referred to as leukocyte interferon, comprises a group of related but distinct proteins that share over 95% amino acid sequence homology. These proteins play a crucial role in the innate immune response against viral infections. The IFN-α family includes 13 subtypes and 23 different variants, with individual proteins exhibiting molecular masses ranging from 19 to 26 kDa and lengths of 156 to 166 and 172 amino acids. All IFN-α subtypes have a conserved sequence region between amino acid positions 115 and 151, while the amino-terminal ends vary. Interferon-alpha 4 (IFNA4) is a member of this alpha interferon family. It is a secreted protein produced by macrophages. There are two known variants of IFNA4, namely IFNA4a, and IFNA4b, which differ at nucleotide positions 220 and 410 in their coding regions. |
