| Source: |
Escherichia coli. |
| Molecular Weight: |
Approximately 19.4 kDa, a single non-glycosylated polypeptide chain containing 166 amino acids. |
| AA Sequence: |
MCDLPQTHSL GSRRTLMLLA QMRRISLFSC LKDRHDFGFP QEEFGNQFQK AETIPVLHEM IQQIFNLFST KDSSAAWDET LLDKFYTELY QQLNDLEACV IQGVGVTETP LMKEDSILAV RKYFQRITLY LKEKKYSPCA WEVVRAEIMR SFSLSTNLQE SLRSKE |
| Purity: |
> 96% by SDS-PAGE and HPLC analyses. |
| Biological Activity: |
Fully biologically active when compared to standard. The specific activity determined by an anti-viral assay is no less than 1.0 × 108 IU/mg. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from a 0.2 µm filtered solution in PBS, pH7.4. |
| Endotoxin: |
Less than 1 EU/µg of rHuIFN-α2b, Ecoli. as determined by LAL method. |
| Reconstitution: |
Centrifuge the vial briefly before opening to ensure that the contents settle at the bottom. Reconstitute the vial with sterile distilled water or an aqueous buffer containing 0.1% BSA to achieve a concentration of 0.1-1.0 mg/mL. Divide the resulting stock solution into working aliquots and store them at or below -20°C. For further dilutions, use appropriate buffered solutions. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Background: |
IFN-αs are proteins exuded by leukocytes and primarily act in the innate immune response to viral infections. The IFN-α family is comprised of 13 subtypes and 23 variants. These proteins have molecular masses ranging from 19-26 kDa, consisting of proteins that are 156-166 or 172 amino acids in length. All IFN-α subtypes feature a conserved sequence region located between positions 115-151 of the amino acid, while their amino-terminal ends vary. Many IFN-α subtypes differ in their sequences at just one or two positions. Natural variants can also include proteins truncated by 10 amino acids at the carboxy-terminal end. |
