| Source: |
Escherichia coli |
| Molecular Weight: |
Approximately 7743 Da, a single non-glycosylated polypeptide chain containing 70 amino acids. 15N stable isotope labeled. |
| AA Sequence: |
GPETLCGAEL VDALQFVCGD RGFYFNKPTG YGSSSRRAPQ TGIVDECCFR SCDLRRLEMY CAPLKPAKSA |
| Purity: |
>97% by SDS-PAGE analyses.
>90% by RP-HPLC analyses. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from a 0.2 µm filtered concentrated solution in PBS, 0.02% Tween-20, pH7.0. |
| Endotoxin: |
Less than 0.1 EU/µg of rHuIGF-1, 15N as determined by LAL method. |
| Reconstitution: |
Prior to opening, it is recommended to centrifuge the vial briefly to bring the contents down the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. If animal-origin-free condition is expected in your product, then sterile distilled water is recommended. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
A minimum of 12 months from date of receipt, when stored at ≤ -20 ℃ as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Background: |
Insulin-like growth factors (IGFs) are mitogenic polypeptide growth factors that belong to the insulin gene family. They promote the proliferation and survival of various cell types, including muscle, bone, and cartilage tissue, in vitro. While structurally and functionally similar to insulin, IGFs exhibit much higher growth-promoting activity. IGF-1, specifically, is primarily produced by the liver as an endocrine hormone, but it is also synthesized in target tissues in a paracrine/autocrine manner. Growth hormone (GH) stimulates IGF-1 production, which can be inhibited by factors such as undernutrition, growth hormone insensitivity, lack of growth hormone receptors, or defects in the downstream signaling pathway post-GH receptor, including SHP2 and STAT5B. Recombinant human IGF-1 proteins are globular, consisting of 70 amino acids and three intramolecular disulfide bonds. Mature human IGF-1 shares 94% and 96% amino acid sequence identity with mouse and rat IGF-1, respectively, and shows cross-species activity. |
