| Source: |
Escherichia coli |
| Molecular Weight: |
Approximately 8.4 kDa, a single non-glycosylated polypeptide chain containing 72 amino acids. |
| AA Sequence: |
TESSSRGPYH PSECCFTYTT YKIPRQRIMD YYETNSQCSK PGIVFITKRG HSVCTNPSDK WVQDYIKDMK EN |
| Purity: |
> 96% by SDS-PAGE and HPLC analyses. |
| Biological Activity: |
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human monocytes is in a concentration of 5.0-20 ng/mL. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH7.4, 100 mM NaCl. |
| Endotoxin: |
Less than 1 EU/μg of rHuHCC-1/CCL14 as determined by LAL method. |
| Reconstitution: |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Synonyms: |
HCC-1/HCC-3, NCC-2, Small-inducible Cytokine A14 |
| Background: |
Human CCL14 belongs to the CC chemokine family and comprises two isoforms: CCL14a (HCC-1) and CCL14b (HCC-3). The sequence of HCC-3 differs from HCC-1 at position 27, where R is replaced by QTGGKPKVVKIQLKLVG. Initially isolated from the hemofiltrate of patients with chronic renal failure, CCL14 promotes chemotaxis of T lymphocytes, monocytes, and eosinophils. It also impedes infection by M-tropic HIV-1 and acts as a ligand for CCR1, CCR3, and CCR5 receptors. CCL14 is constitutively expressed in various normal tissues including the spleen, liver, skeletal muscle, heart muscle, gut, and bone marrow, and is found at high concentrations in plasma. |
