| Source: |
Escherichia coli. |
| Molecular Weight: |
Approximately 7.9 kDa, a single non-glycosylated polypeptide chain containing 73 amino acids. |
| AA Sequence: |
ASVATELRCQ CLQTLQGIHP KNIQSVNVKS PGPHCAQTEV IATLKNGRKA CLNPASPIVK KIIEKMLNSD KSN |
| Purity: |
> 97% by SDS-PAGE and HPLC analyses. |
| Biological Activity: |
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood neutrophils is in a concentration range of 10-50 ng/mL. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from a 0.2 µm filtered concentrated solution in 20 mM PB, pH7.4, 150 mM NaCl. |
| Endotoxin: |
Less than 1 EU/µg of rHuGRO-α/CXCL1 as determined by LAL method. |
| Reconstitution: |
Centrifuge the vial briefly before opening to ensure that the contents settle at the bottom. Reconstitute the vial with sterile distilled water or an aqueous buffer containing 0.1% BSA to achieve a concentration of 0.1-1.0 mg/mL. Divide the resulting stock solution into working aliquots and store them at or below -20°C. For further dilutions, use appropriate buffered solutions. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Synonyms: |
GROα, MGSA, NAP-3 |
| Background: |
CXCL1, part of the CXC chemokine family, is produced by the GRO gene, now designated as CXCL1. Initially identified in hamster cells, CXCL1 shares 90% and 86% amino acid sequence identity with GROβ and GROγ, respectively. All three human GROs belong to the intercrine alpha subfamily of chemokines. CXCL1 is secreted by various cell types, including human melanoma cells, macrophages, neutrophils, and epithelial cells, and binds to the CXCR2 receptor. It has chemotactic activity for neutrophils and plays roles in inflammation and wound healing. |
